Isolation and culture of human primary keratinocytes – a methods review

review of radiographic methods in dental implantology

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In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold

Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Hepatocyte Isolation and Culture Methods

The liver plays a major role in in vivo metabolisms such as in synthesis of plasma proteins, blood glucose generation, urea formation, secretion of bile, and detoxification. Since most of these hepatic functions are performed by hepatocytes, parenchymal cells of the liver, there has been much attention to their use for cell therapeutic applications and the in vitro studies of liver physiology, ...

Osteocyte isolation and culture methods.

The aim of this paper is to present several popular methods for in vitro culture of osteocytes and osteocyte cell lines. Osteocytes are located extremely suitably within the calcified bone matrix to sense mechanical signals, and are equipped with a multitude of molecular features that allow mechanosensing. However, osteocytes are more than specialized mechanosensing cells. Several signaling mol...